Phosphinothricin-resistant
WebJan 18, 2024 · One of the microbes that produce phosphinothricin, Streptomyces … WebOct 23, 2015 · L-Phosphinothricin [PPT; glufosinate; L-homoalanine-4-yl- (methyl)phosphinic acid] is the active ingredient of the nonselective herbicide BASTA (AgrEvo, Frankfurt am Main, Germany), which is a nonselective, postemergence herbicide used for weed control in orchards and vineyards 1.
Phosphinothricin-resistant
Did you know?
WebNov 6, 2006 · Selection for kanamycin-, phosphinothricin- and hygromycin B-resistance commonly takes 7-10 d and high seedling density and fungal contamination may result in failure to recover transformants. Results: A method for identifying transformed seedlings in as little as 3.25 d has been developed. WebBialaphos resistance (BAR) and phosphinothricin acetyltransferase (PAT) genes, which convey resistance to the broad-spectrum herbicide phosphinothricin (also known as glufosinate) via N-acetylation, have been globally used in basic plant research and genetically engineered crops1–4.
WebOct 4, 2009 · The transgenic plants were resistant to phosphinothricin up to 200 mg/l, … WebJan 27, 2024 · The fast spread of bacteria that are resistant to many classes of antibiotics (multidrug resistant) is a global threat to human and animal health with a worrisome scenario ahead. Novel therapeutical strategies are …
WebJan 1, 2008 · The use of phosphinothricin resistance as selectable marker for genetic . transformation of grapevine. R. J ARDAK-J AMOUSSI 1), B. B OUAMAMA 1), A. M LIKI 1), A. G HORBEL 1) and G. M. R EUSTLE 2) WebThe present invention relates to specific herbicide combinations comprising (i) L-glufosinate and/or salts thereof and (ii) carfentrazone-ethyl in specific ratios, and to compositions comprising said herbicide combinations in these ratios. The present invention further relates to a method of producing said specific herbicide combinations and compositions …
Webresistance of expiants to, phosphinothricin is presented. Key words: Ammonium - Carbon source - Glufosinate - Glutamine - Metabolic activity - Phosphinothricin Introduction Selectable marker genes are still a necessity in plant trans formation technology. In the presence of a selective agent, only those cells that are transformed and express the
WebUnder optimised electroporation conditions, up to 0.9% of viable protoplasts displayed gus activity two days after electroporation. To select for phosphinothricin (PPT) resistant colonies, electroporated protoplasts were incubated for six weeks in a medium containing 10 μg/ml PPT. The cells surviving the selection were maintained as individual ... chinti and parker jumperWebOct 21, 2024 · The CRISPR vector for this study was used in a previously published pBAtC that is composed of the U6 promoter from Arabidopsis to express gRNA including CRISPR RNA scaffold, the 35S promoter originally from cauliflower mosaic virus (CaMV) to express Cas9 and the Basta TM (Phosphinothricin) resistance gene for the selection of the first ... chinti and parker maxi dressGlufosinate (also known as phosphinothricin and often sold as an ammonium salt) is a naturally occurring broad-spectrum herbicide produced by several species of Streptomyces soil bacteria. Glufosinate is a non-selective, contact herbicide, with some systemic action. Plants may also metabolize bialaphos and phosalacine, other naturally occurring herbicides, directly into glufosinate. The … granny\\u0027s sandwich shopWebA bioassay was developed for identifying transgenic wheat plants based on De Block et … granny\u0027s restaurant in cherokee ncWebPhosphinothricin is not an effective antibiotic because of its inability to be readily … chinti and parker returnsWebJan 1, 2010 · The resistance to phosphinothricin is stable and is inherited by the R1 progeny as a single dominant Mendelian trait. The transgenic plants are highly resistant to the broad spectrum herbicide ... granny\u0027s rocking chair double deckersWebResistance plasmids include pGreenII 0229 and pGreenII 0229 62-SK. pGreenII 0229 is derived from pGreenII 0000, a nos-bar cassette has been inserted into the HpaI site of the left border, providing resistance to bialaphos or phosphinothricin during plant transformation selection. pGreenII 0229 62-SK is derived from pGreenII 0229, the LacZ … granny\\u0027s salisbury steak