Klf2 chip

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August undefined, 2024

Webb8 mars 2024 · KLF2 and KLF3 repressed HIV-1 and HIV-2 transcription in CD4+ T cells, whereas PLAGL1 activated transcription of HIV-2 through direct protein–DNA interactions. Using computational modeling with interacting proteins, we leveraged the results from our screen to identify putative pathways that define intrinsic transcriptional networks. Webb25 apr. 2006 · Mechanistically, KLF2 inhibits the transcriptional activity of both NF-κB and activator protein 1, in part by means of recruitment of transcriptional coactivator …

Myeloid Krüppel-like factor 2 is a critical regulator of ... - Nature

Webb6 apr. 2012 · Overlap of Published Klf2 ChIP-on-Chip Targets with Prdm14 and Stat3 ChIP-Seq Targets Request PDF. Table S4. Overlap of Published Klf2 ChIP-on-Chip … Webb20 juli 2006 · KLF2 is expressed in lung, endothelial cells and lymphocytes 4, 8, 9, 10, 11, and is essential for normal blood-vessel integrity and lung development 4, 9, 10, 11. … def of release

Kruppel-Like Factor 2 (KLF2) Regulates ... - Circulation Research

Webb13 apr. 2012 · RA induces the expression of the adipogenesis inhibitors Pref-1, Sox9, and KLF2. A: Levels of FABP4 mRNA in cells differentiated in the presence of RA, LE540, or both (1 μmol/L). * P ≤ 0.01 control versus treated. B: Levels of RARα ( ), RARγ ( ), and CRABP-II ( ) mRNA of during the first 24 h of adipocyte differentiation. WebbKlf2 activates transcription through its binding of the beta-globin gene promoter CACCC box. Studies have shown Klf2 to have critical roles in both the function and … Webb31 jan. 2013 · KLF2 is essential for and contributes significantly to statin enhancement of tBHQ-induced γ-globin gene expression. K562 cells were transiently transfected with siRNA specific for KLF2. Twenty-four hours later, cells were treated with tBHQ or tBHQ and simvastatin (statin) for 48 hours before RNA isolation. fe ministry\u0027s

Identifying a novel KLF2/lncRNA SNHG12/miR-494-3p/RAD23B …

KFA2

WebbChIP was conducted using a ChIP assay kit (Cell Signaling Technology, USA) as described previously (Cui et al., 2024). For the KLF2 ChIP assay, DF1 cells were transfected with WebbFurthermore, KLF2-ChIP analysis revealed that during osteoclastic differentiation KLF2 binding was decreased on Becn1 promoter regions. These data confirm that KLF2 expression was not only reduced in differentiated osteoclast cells but also binding efficiency of KLF2 was decreased on Becn1 promoter, which resulted in activation of Becn1 gene … def of reliableWebb21 mars 2024 · KLF2 (KLF Transcription Factor 2) is a Protein Coding gene. Diseases associated with KLF2 include Cerebral Cavernous Malformations and Spleen Cancer. … def of relief

"WebbChIP assays were performed using anti-Klf2, anti-Klf4 and anti-Klf5 antibodies with extracts derived from ES cells transfected with control RNAi construct (grey bar) or ES cells transfected... " - Klf2 chip

Klf2 chip

A core Klf circuitry regulates self-renewal of embryonic stem cells

Webb2 feb. 2024 · For the KLF2 ChIP assay, DF1 cells were transfected with pCMV-Myc-KLF2 or an empty pCMV-Myc vector. The transfected cells were fixed in 1% formaldehyde for … WebbThe regulation of KLF2 by miR-92a-3p was verified by a series of in vitro and in vivo assays. Further exploration revealed that Baculoviral IAP Repeat Containing 5 (BIRC5) was the target of KLF2. ChIP assay, dual-luciferase reporter analysis, quantitative real-time PCR, and western blot were performed for verification. Results

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Webb18 nov. 2024 · KLF2 regulates metaflammation transcription in macrophages RNA-sequencing of bone marrow-derived macrophages (BMDMs) isolated from myeloid-specific KLF2 knockout (K2KO) and control (LysM) mice... Webb15 juli 2011 · KLF2 is a positive regulator of the mouse and human embryonic β-globin genes . KLF1 and KLF2 have high homology within their DNA-binding domains and …

WebbKLF2 has important roles in embryonic and adult vasculogenesis and angiogenesis under physiological and pathological conditions and plays a key role in embryonic heart valve … Webb31 jan. 2013 · KLF2 is essential for and contributes significantly to statin enhancement of tBHQ-induced γ-globin gene expression. K562 cells were transiently transfected with siRNA specific for KLF2. Twenty-four hours later, cells were treated with tBHQ or tBHQ and simvastatin (statin) for 48 hours before RNA isolation.

WebbF, Chromatin immunoprecipitation assays verify KLF2 binding to the TM promoter. ChIP assays were performed as described in methods using extracts from HUVECs infected with control (Ad-GFP) or Flag-KLF2 (Ad-KLF2). β-Actin is used as a control. Examination of the thrombomodulin promoter revealed the presence of numerous potential binding sites. WebbKLF2 (kruppel-like factor 2 [lung]) regulates osteoclastogenesis by modulating autophagy Macroautophagy/autophagy is involved in myeloid cellular repair, destruction, and …

Webb13 okt. 2024 · Krüppel-like factor 2 (KLF2), a transcription factor of the krüppel-like family, is a key regulator of activation, differentiation, and migration processes in various cell types. In this review, we focus on the functional relevance of KLF2 in immune cell migration and homing. We summarize the key fu …

Webb1 sep. 2024 · KLF2 kruppel-like factor 2 (lung) MAP1LC3B/LC3B microtubule-associated protein 1 light chain 3 beta MDC monodansylcadaverine mTOR mammalian target of … def of reliabilityWebb12 jan. 2024 · Chromatin immunoprecipitation (ChIP) and PCR To investigate the interaction between S1PR1 promoter and transcription factor KLF2, ChIP assay was performed with EZ-Magna ChIP A/G kit (EMD Millipore, Billerica, MA, USA) in accordance with the manufacturer’s procedure. Brieﬂy, EC cells were ﬁxed by 1% formaldehyde, … feminist robotWebbKLF2 is a positive regulator of the mouse and human embryonic β-globin genes. KLF1 and KLF2 have highly homologous zinc finger DNA-binding domains. They have … def of relicWebbKLF2 belongs to the krueppel C2H2-type zinc-finger protein family. It contains 3 C2H2-type zinc fingers. KLF2 binds to the CACCC box in the beta-globin gene promoter and … def of relevanceWebb23 aug. 2024 · KLF siRNA reduced KLF2 and KLF4 gene expression levels under LSS by 79.7% and 69.8%, respectively (Supplementary Fig. 4c ). We note that this still corresponds to 4.9- and 7.8-fold higher gene... feminist roastsWebbAntibodies that detect KLF2 can be used in several scientific applications, including Immunohistochemistry, Western Blot, Immunocytochemistry, Flow Cytometry and ChIP. … feministryWebbAnti-KLF2 antibodies are offered by a number of suppliers. This target gene encodes the protein 'Kruppel like factor 2' in humans and may also be known as LKLF, Kruppel-like Factor 2, Krueppel-like factor 2, and Kruppel-like factor 2 (lung). Structurally, the protein is reported to be 37.4 kilodaltons in mass. feminists affairs